Publications

Publications, Books, Book Chapters and Reviews by Prof. Marcus Maurer, MD

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In vivo non-invasive staining-free visualization of dermal mast cells in healthy, allergy and mastocytosis humans using two-photon fluorescence lifetime imaging

Filename 399. Kröger et al., in vivo non-invasive staining-free visualization MC, SciRep2020.pdf
Filesize 2.43 MB
Version o.399
Date added October 12, 2020
Downloaded 6 times
Category Original Work
Tags mast cells
Authors Kröger, M., Scheffel, J., Nikolaev, V. V., Shirshin, E. A., Siebenhaar, F., Schleusener, J., Lademann, J., Maurer, M., and Darvin, M. E.
Citation Kröger, M., Scheffel, J., Nikolaev, V. V., Shirshin, E. A., Siebenhaar, F., Schleusener, J., Lademann, J., Maurer, M., and Darvin, M. E.: In vivo non-invasive staining-free visualization of dermal mast cells in healthy, allergy and mastocytosis humans using two-photon fluorescence lifetime imaging. Sci. Rep. 2020: 10; 14930.
Corresponding authors Darvin, M. E.
DocNum o.399
DocType PDF
IF 4.38
Publisher Sci. Rep.
ReleaseDate 2020

Mast cells (MCs) are multifunctional cells of the immune system and are found in skin and all major tissues of the body. They contribute to the pathology of several diseases including urticaria, psoriasis, atopic dermatitis and mastocytosis where they are increased at lesional sites. Histomorphometric analysis of skin biopsies serves as a routine method for the assessment of MC numbers and their activation status, which comes with major limitations. As of now, non-invasive techniques to study MCs in vivo are not available. Here, we describe a label-free imaging technique to visualize MCs and their activation status in the human papillary dermis in vivo. This technique uses two-photon excited fluorescence lifetime imaging (TPE-FLIM) signatures, which are different for MCs and other dermal components. TPE-FLIM allows for the visualization and quantification of dermal MCs in healthy subjects and patients with skin diseases. Moreover, TPE-FLIM can differentiate between two MC populations in the papillary dermis in vivo—resting and activated MCs with a sensitivity of 0.81 and 0.87 and a specificity of 0.85 and 0.84, respectively. Results obtained on healthy volunteers and allergy and mastocytosis patients indicate the existence of other MC subpopulations within known resting and activated MC populations. The developed method may become an important tool for noninvasive in vivo diagnostics and therapy control in dermatology and immunology, which will help to better understand pathomechanisms involving MC accumulation, activation and degranulation and to characterize the effects of therapies that target MCs.

 

(Last update: 12.2023)

Number of original publications in peer-reviewed journals:580
Number of reviews in peer-reviewed journals:210
Number of publications (original work and reviews) in peer-reviewed journals:790
Cumulative IF for original publications in peer-reviewed journals:4196.39
Cumulative IF for reviews in peer-reviewed journals:1409.32
Cumulative IF of publications (original work & reviews) in peer-reviewed journals:5605.71
Total number of citations: 36,836, h-index: 99 (Web of Science December 2023)36836

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