Publications
Publications, Books, Book Chapters and Reviews by Prof. Marcus Maurer, MD
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Basophilic histamine content and release during venom immunotherapy: insights by flow cytometry
Filename | 159. Nullens et al., Basophilic hist. content,CYTOM.CLIN.2013.pdf |
Filesize | 338.14 KB |
Version | o.159 |
Date added | June 5, 2020 |
Downloaded | 4 times |
Category | Original Work |
Tags | allergen immunotherapies, basophil, flow cytometry, histamine |
Authors | Nullens, S., Sabato, V., Faber, M., Leysen, J., Bridts, C. H., de Clerck, L. S., Falcone, F., Maurer, M. and Ebo, D. G. |
Citation | Nullens, S., Sabato, V., Faber, M., Leysen, J., Bridts, C. H., de Clerck, L. S., Falcone, F., Maurer, M.and Ebo, D. G.: Basophilic histamine content and release during venom immunotherapy: insights by flow cytometry. Cytom. Part B – Clin. CY 2013: 84; 173-178. |
Corresponding authors | Ebo, D. G. |
DocNum | O.159 |
DocType | |
Edition; Page | 84; 173-178 |
IF | 2.28 |
Publisher | Cytom. Part B – Clin. CY |
ReleaseDate | 2013 |
Background: Despite the efficiency of venom immunotherapy, the effects on basophils and mast cells remain incompletely understood and probably vary according to the treatment phase.
Objectives: To study the effect of build-up and maintenance venom immunotherapy on individual basophils.
Methods: Intracellular histamine and its release was analyzed flow cytometrically by a new enzyme-af- finity method using diamine oxidase conjugated to laser-excitable fluorochromes.
Phenotyping of cells included flow cytometric quantification of CD63 and CD203c. Analyses of basophil activation experiments were performed before the start of treatment, after build-up therapy and during maintenance therapy.
Results: Before the start of therapy, patients demonstrated significantly higher numbers of basophils when compared with stung control individuals. At the end of build-up therapy a decrease of basophil num- bers was observed, whereas during maintenance therapy basophil counts returned to pretreatment values. Before the start of therapy, the intracellular histamine content per cell in patients was significantly higher when compared with stung control individuals. During maintenance therapy intracellular histamine content decreased to values observed in stung control individuals. In addition, maintenance therapy lowered the net release of histamine per cell in response to optimal stimulation with wasp venom.
Conclusions: We introduce a novel technique that enables to assess the effects of venom immunother- apy on basophils. This new technique may help to monitor treatment effects in individual patients and could aid in the development of more efficient and better tolerated immunotherapy protocols.
(Last update: 12.2023)
Number of original publications in peer-reviewed journals: | 580 |
Number of reviews in peer-reviewed journals: | 210 |
Number of publications (original work and reviews) in peer-reviewed journals: | 790 |
Cumulative IF for original publications in peer-reviewed journals: | 4196.39 |
Cumulative IF for reviews in peer-reviewed journals: | 1409.32 |
Cumulative IF of publications (original work & reviews) in peer-reviewed journals: | 5605.71 |
Total number of citations: 36,836, h-index: 99 (Web of Science December 2023) | 36836 |
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